Journal: American Journal of Translational Research

Article Title: LncRNA AL645608.3 mediates malignant progression of acute myeloid leukemia

doi:

Figure Lengend Snippet: Primer information for RT-qPCR

Article Snippet: The transferred membranes were blocked for 1 h. After discarding the sealing liquid, the diluted primary antibodies against IRF6 (#sc-37704; 1:500; Santa, Texas, USA), CBL (#25818-1-AP; 1:1000; Proteintech, Wuhan, China), IFNB1 (#ab275880; 1:500; Abcam, Massachusetts, USA), and GAPDH (#ab181602; 1:10000; Abcam) were added and incubated overnight at 4°C.

Techniques: Sequencing

Journal: American Journal of Translational Research

Article Title: LncRNA AL645608.3 mediates malignant progression of acute myeloid leukemia

doi:

Figure Lengend Snippet: Effects of AL645608.3 on the mRNA expression of IRF6, CBL and IFNB1 in AML cells. A-C. The mRNA expression of IRF6, CBL and IFNB1 in THP-1 cells infected with sh-AL645608.3 lentiviruses. D-F. The mRNA expression of IRF6, CBL and IFNB1 in AML-193 cells infected with AL645608.3 overexpression lentiviruses. ns, P>0.05; ****P<0.0001.

Article Snippet: The transferred membranes were blocked for 1 h. After discarding the sealing liquid, the diluted primary antibodies against IRF6 (#sc-37704; 1:500; Santa, Texas, USA), CBL (#25818-1-AP; 1:1000; Proteintech, Wuhan, China), IFNB1 (#ab275880; 1:500; Abcam, Massachusetts, USA), and GAPDH (#ab181602; 1:10000; Abcam) were added and incubated overnight at 4°C.

Techniques: Expressing, Infection, Over Expression

Journal: American Journal of Translational Research

Article Title: LncRNA AL645608.3 mediates malignant progression of acute myeloid leukemia

doi:

Figure Lengend Snippet: Effects of AL645608.3 on the protein expression of IRF6, CBL and IFNB1 in AML cells. A. Representative western blot images for IRF6, CBL and IFNB1 in THP-1 cells with sh-AL645608.3 lentiviruses infection and in AML-193 cells with AL645608.3 overexpression lentiviruses infection. B-D. Western blot for the detection of protein levels of IRF6, CBL and IFNB1 in THP-1 cells infected with sh-AL645608.3 lentiviruses. E-G. Western blot for the detection of protein levels of IRF6, CBL and IFNB1 in AML-193 cells infected with AL645608.3 overexpression lentiviruses. ns, P>0.05; **P<0.01; ***P<0.001; ****P<0.0001.

Article Snippet: The transferred membranes were blocked for 1 h. After discarding the sealing liquid, the diluted primary antibodies against IRF6 (#sc-37704; 1:500; Santa, Texas, USA), CBL (#25818-1-AP; 1:1000; Proteintech, Wuhan, China), IFNB1 (#ab275880; 1:500; Abcam, Massachusetts, USA), and GAPDH (#ab181602; 1:10000; Abcam) were added and incubated overnight at 4°C.

Techniques: Expressing, Western Blot, Infection, Over Expression

Journal: Journal of Neuroinflammation

Article Title: Activation of dopamine D1 receptor decreased NLRP3-mediated inflammation in intracerebral hemorrhage mice

doi: 10.1186/s12974-017-1039-7

Figure Lengend Snippet: Expressions of DRD1 and IFN-beta receptor in microglia. a Co-localization of DRD1 with Iba 1 and of IFN-beta receptor with Iba 1 was observed in the perihematoma brain tissue (showing with black triangle) at 24 h after ICH. b Representative photographs of co-localization of DRD1 with Iba 1 (bar = 50 μm). c Representative photographs of co-localization of IFN-beta receptor with Iba 1 (bar = 50 μm)

Article Snippet: After blocking with 5% nonfat milk for 1.5 h, the membranes were incubated overnight at 4 °C with the primary antibodies: rabbit anti-DRD1 (1:1000, Abcam), goat anti-IFN-beta (1:300, Santa Cruz), rabbit anti-IFN-beta receptor (1:500, Santa Cruz), rabbit anti-STAT1(1:2000, Cell Signaling), goat anti-p-STAT1 (1:500, Santa Cruz), rabbit anti-NLRP3 (1:1000, MyBioSource), rabbit anti-caspase1 (1:1000, NOVUS Biologicals), rabbit anti-IL-1beta (1:2000, Cell Signaling), and rabbit anti-β-actin (1:2000, Santa Cruz). β-actin served as the loading control.

Techniques:

Journal: Journal of Neuroinflammation

Article Title: Activation of dopamine D1 receptor decreased NLRP3-mediated inflammation in intracerebral hemorrhage mice

doi: 10.1186/s12974-017-1039-7

Figure Lengend Snippet: Expressions of DRD1 and IFN-beta in the ipsilateral hemispheres after intracerebral hemorrhage (ICH). a Representative photographs of DRD1 and IFN-beta in western blotting. b Bar graphs of quantitative analysis of DRD1 and IFN-beta expressions from the ipsilateral hemisphere after ICH. DRD1 and IFN-beta expressions increased, * p < 0.05, ** p < 0.01 versus sham; DRD1 and IFN-beta expressions decreased, # p < 0.05, ## p < 0.01 versus sham

Article Snippet: After blocking with 5% nonfat milk for 1.5 h, the membranes were incubated overnight at 4 °C with the primary antibodies: rabbit anti-DRD1 (1:1000, Abcam), goat anti-IFN-beta (1:300, Santa Cruz), rabbit anti-IFN-beta receptor (1:500, Santa Cruz), rabbit anti-STAT1(1:2000, Cell Signaling), goat anti-p-STAT1 (1:500, Santa Cruz), rabbit anti-NLRP3 (1:1000, MyBioSource), rabbit anti-caspase1 (1:1000, NOVUS Biologicals), rabbit anti-IL-1beta (1:2000, Cell Signaling), and rabbit anti-β-actin (1:2000, Santa Cruz). β-actin served as the loading control.

Techniques: Western Blot

Journal: Journal of Neuroinflammation

Article Title: Activation of dopamine D1 receptor decreased NLRP3-mediated inflammation in intracerebral hemorrhage mice

doi: 10.1186/s12974-017-1039-7

Figure Lengend Snippet: Effects of A68930 on the expressions of proteins in DRD1/IFN-beta/STAT1/NLRP3 pathway after ICH. a Representative photographs of the expressions of proteins in DRD1/IFN-beta/STAT1/NLRP3 pathway in western blotting. b and c Bar graphs of quantitative analysis of DRD1, IFN-beta, IFN-beta receptor, p-STAT1, NLRP3, pro-caspase1, caspase1, pro-IL-1beta, and IL-1beta, respectively. * p < 0.05, ** p < 0.01 versus sham; # p < 0.05, ## p < 0.01 versus vehicle

Article Snippet: After blocking with 5% nonfat milk for 1.5 h, the membranes were incubated overnight at 4 °C with the primary antibodies: rabbit anti-DRD1 (1:1000, Abcam), goat anti-IFN-beta (1:300, Santa Cruz), rabbit anti-IFN-beta receptor (1:500, Santa Cruz), rabbit anti-STAT1(1:2000, Cell Signaling), goat anti-p-STAT1 (1:500, Santa Cruz), rabbit anti-NLRP3 (1:1000, MyBioSource), rabbit anti-caspase1 (1:1000, NOVUS Biologicals), rabbit anti-IL-1beta (1:2000, Cell Signaling), and rabbit anti-β-actin (1:2000, Santa Cruz). β-actin served as the loading control.

Techniques: Western Blot

Journal: Journal of Neuroinflammation

Article Title: Activation of dopamine D1 receptor decreased NLRP3-mediated inflammation in intracerebral hemorrhage mice

doi: 10.1186/s12974-017-1039-7

Figure Lengend Snippet: DRD1 antagonist and IFN-beta siRNA reversed effects of A68930 on neurological outcome and brain water content after ICH. Garcia test score ( a ), left forelimb placement ( b ), and brain water content ( c ) at 24 h after ICH. ** p < 0.01 versus sham; # p < 0.05, ## p < 0.01 versus vehicle; $ p < 0.05, $$ p < 0.01 versus A68930 group; & p < 0.05 versus scramble siRNA group

Article Snippet: After blocking with 5% nonfat milk for 1.5 h, the membranes were incubated overnight at 4 °C with the primary antibodies: rabbit anti-DRD1 (1:1000, Abcam), goat anti-IFN-beta (1:300, Santa Cruz), rabbit anti-IFN-beta receptor (1:500, Santa Cruz), rabbit anti-STAT1(1:2000, Cell Signaling), goat anti-p-STAT1 (1:500, Santa Cruz), rabbit anti-NLRP3 (1:1000, MyBioSource), rabbit anti-caspase1 (1:1000, NOVUS Biologicals), rabbit anti-IL-1beta (1:2000, Cell Signaling), and rabbit anti-β-actin (1:2000, Santa Cruz). β-actin served as the loading control.

Techniques:

Journal: Journal of Neuroinflammation

Article Title: Activation of dopamine D1 receptor decreased NLRP3-mediated inflammation in intracerebral hemorrhage mice

doi: 10.1186/s12974-017-1039-7

Figure Lengend Snippet: DRD1 antagonist and IFN-beta siRNA reversed effects of A68930 on DRD1/IFN-beta/STAT1/NLRP3 signaling pathway after ICH. a Representative photographs of the expressions of proteins in DRD1/IFN-beta/STAT1/NLRP3 pathway in western blotting. b and c Bar graphs of quantitative analysis of DRD1, IFN-beta, IFN-beta receptor, p-STAT1, NLRP3, pro-caspase1, caspase1, pro-IL-1beta, and IL-1beta, respectively. * p < 0.05, ** p < 0.01 versus sham; # p < 0.05, ## p < 0.01 versus vehicle; $ p < 0.05, $$ p < 0.01 versus A68930 group; & p < 0.05, && p < 0.01 versus scramble siRNA group

Article Snippet: After blocking with 5% nonfat milk for 1.5 h, the membranes were incubated overnight at 4 °C with the primary antibodies: rabbit anti-DRD1 (1:1000, Abcam), goat anti-IFN-beta (1:300, Santa Cruz), rabbit anti-IFN-beta receptor (1:500, Santa Cruz), rabbit anti-STAT1(1:2000, Cell Signaling), goat anti-p-STAT1 (1:500, Santa Cruz), rabbit anti-NLRP3 (1:1000, MyBioSource), rabbit anti-caspase1 (1:1000, NOVUS Biologicals), rabbit anti-IL-1beta (1:2000, Cell Signaling), and rabbit anti-β-actin (1:2000, Santa Cruz). β-actin served as the loading control.

Techniques: Western Blot

Journal: eLife

Article Title: LRRK2 maintains mitochondrial homeostasis and regulates innate immune responses to Mycobacterium tuberculosis

doi: 10.7554/eLife.51071

Figure Lengend Snippet:

Article Snippet: Antibody , anti-IFNB Rabbit polyclonal , PBL Assay Science , 32400–1 , (1:250 neutralizing).

Techniques: Sequencing, Recombinant

Journal: Journal of Neuroinflammation

Article Title: Activation of dopamine D1 receptor decreased NLRP3-mediated inflammation in intracerebral hemorrhage mice

doi: 10.1186/s12974-017-1039-7

Figure Lengend Snippet: Expressions of DRD1 and IFN-beta receptor in microglia. a Co-localization of DRD1 with Iba 1 and of IFN-beta receptor with Iba 1 was observed in the perihematoma brain tissue (showing with black triangle) at 24 h after ICH. b Representative photographs of co-localization of DRD1 with Iba 1 (bar = 50 μm). c Representative photographs of co-localization of IFN-beta receptor with Iba 1 (bar = 50 μm)

Article Snippet: A series of coronal brain sections (10 μm thick) were blocked in 5% bovine serum albumin for 2 h at room temperature and then were incubated with rabbit anti-DRD1 (1:200, Abcam), rabbit anti-IFN-beta receptor (1:50, Santa Cruz), goat anti-ionized calcium-binding adapter molecule 1 (Iba 1, 1:200, Abcam), and rabbit anti-MPO (1:100, Abcam) at 4 °C overnight.

Techniques:

Journal: Journal of Neuroinflammation

Article Title: Activation of dopamine D1 receptor decreased NLRP3-mediated inflammation in intracerebral hemorrhage mice

doi: 10.1186/s12974-017-1039-7

Figure Lengend Snippet: Expressions of DRD1 and IFN-beta in the ipsilateral hemispheres after intracerebral hemorrhage (ICH). a Representative photographs of DRD1 and IFN-beta in western blotting. b Bar graphs of quantitative analysis of DRD1 and IFN-beta expressions from the ipsilateral hemisphere after ICH. DRD1 and IFN-beta expressions increased, * p < 0.05, ** p < 0.01 versus sham; DRD1 and IFN-beta expressions decreased, # p < 0.05, ## p < 0.01 versus sham

Article Snippet: A series of coronal brain sections (10 μm thick) were blocked in 5% bovine serum albumin for 2 h at room temperature and then were incubated with rabbit anti-DRD1 (1:200, Abcam), rabbit anti-IFN-beta receptor (1:50, Santa Cruz), goat anti-ionized calcium-binding adapter molecule 1 (Iba 1, 1:200, Abcam), and rabbit anti-MPO (1:100, Abcam) at 4 °C overnight.

Techniques: Western Blot

Journal: Journal of Neuroinflammation

Article Title: Activation of dopamine D1 receptor decreased NLRP3-mediated inflammation in intracerebral hemorrhage mice

doi: 10.1186/s12974-017-1039-7

Figure Lengend Snippet: Effects of A68930 on the expressions of proteins in DRD1/IFN-beta/STAT1/NLRP3 pathway after ICH. a Representative photographs of the expressions of proteins in DRD1/IFN-beta/STAT1/NLRP3 pathway in western blotting. b and c Bar graphs of quantitative analysis of DRD1, IFN-beta, IFN-beta receptor, p-STAT1, NLRP3, pro-caspase1, caspase1, pro-IL-1beta, and IL-1beta, respectively. * p < 0.05, ** p < 0.01 versus sham; # p < 0.05, ## p < 0.01 versus vehicle

Article Snippet: A series of coronal brain sections (10 μm thick) were blocked in 5% bovine serum albumin for 2 h at room temperature and then were incubated with rabbit anti-DRD1 (1:200, Abcam), rabbit anti-IFN-beta receptor (1:50, Santa Cruz), goat anti-ionized calcium-binding adapter molecule 1 (Iba 1, 1:200, Abcam), and rabbit anti-MPO (1:100, Abcam) at 4 °C overnight.

Techniques: Western Blot

Journal: Journal of Neuroinflammation

Article Title: Activation of dopamine D1 receptor decreased NLRP3-mediated inflammation in intracerebral hemorrhage mice

doi: 10.1186/s12974-017-1039-7

Figure Lengend Snippet: DRD1 antagonist and IFN-beta siRNA reversed effects of A68930 on neurological outcome and brain water content after ICH. Garcia test score ( a ), left forelimb placement ( b ), and brain water content ( c ) at 24 h after ICH. ** p < 0.01 versus sham; # p < 0.05, ## p < 0.01 versus vehicle; $ p < 0.05, $$ p < 0.01 versus A68930 group; & p < 0.05 versus scramble siRNA group

Article Snippet: A series of coronal brain sections (10 μm thick) were blocked in 5% bovine serum albumin for 2 h at room temperature and then were incubated with rabbit anti-DRD1 (1:200, Abcam), rabbit anti-IFN-beta receptor (1:50, Santa Cruz), goat anti-ionized calcium-binding adapter molecule 1 (Iba 1, 1:200, Abcam), and rabbit anti-MPO (1:100, Abcam) at 4 °C overnight.

Techniques:

Journal: Journal of Neuroinflammation

Article Title: Activation of dopamine D1 receptor decreased NLRP3-mediated inflammation in intracerebral hemorrhage mice

doi: 10.1186/s12974-017-1039-7

Figure Lengend Snippet: DRD1 antagonist and IFN-beta siRNA reversed effects of A68930 on DRD1/IFN-beta/STAT1/NLRP3 signaling pathway after ICH. a Representative photographs of the expressions of proteins in DRD1/IFN-beta/STAT1/NLRP3 pathway in western blotting. b and c Bar graphs of quantitative analysis of DRD1, IFN-beta, IFN-beta receptor, p-STAT1, NLRP3, pro-caspase1, caspase1, pro-IL-1beta, and IL-1beta, respectively. * p < 0.05, ** p < 0.01 versus sham; # p < 0.05, ## p < 0.01 versus vehicle; $ p < 0.05, $$ p < 0.01 versus A68930 group; & p < 0.05, && p < 0.01 versus scramble siRNA group

Article Snippet: A series of coronal brain sections (10 μm thick) were blocked in 5% bovine serum albumin for 2 h at room temperature and then were incubated with rabbit anti-DRD1 (1:200, Abcam), rabbit anti-IFN-beta receptor (1:50, Santa Cruz), goat anti-ionized calcium-binding adapter molecule 1 (Iba 1, 1:200, Abcam), and rabbit anti-MPO (1:100, Abcam) at 4 °C overnight.

Techniques: Western Blot